Serine Protease Purification Protocol

Serine proteases are a rich variety of enzymes, named after the catalytic triplet consisting of serine, histidine, and aspartic acid, including serine, at the catalytic active site of the enzyme. Some serine proteases, such as thrombin proteases, include thrombin, tissue plasminogen activator, and plasmin, which are involved in coagulation and inflammatory responses; some serine proteases such as trypsin The enzymes involved in digestion include trypsin, elastase, and chymotrypsin; there are also some serine proteases expressed in the nervous system, which are involved in the normal maintenance of the nervous system or mediate the occurrence of pathological conditions.

Benzamidine Affinity Media

Para-aminobenzamidine is not only an important chemical raw material, a pharmaceutical intermediate, but also an inhibitor of enzymes such as trypsin and urokinase. It can also be used as a ligand in affinity chromatography and a probe for the active site of serine protease. Much attention. Welch Benzamidine Tanrose 4FF is an affinity chromatography medium prepared by coupling para-aminobenzamidine to agarose gel and can be used to purify serine proteases.

Welch Benzamidine Tanrose 4FF packing materials

Benzamidine Tanrose 4FF Technical Parameters

Applications

Chromatography column: PreCot Benzamidine 4FF (5mL prepacked column).

Sample: trypsin mixture;

Binding buffer: 50mM PB, 50mM NaCl pH7.0;

Elution buffer: 0.1M GIy pH 2.7 (200μl 1M Tris-HCl pH9.0 was pre-added to the eluate collection tube);

Flow rate: 0.7 mL/min.

Order information